RTA P. jirovecii Real—Time PCR Kit is an in vitro nucleic acid ampliﬁcation assay for the quantitation of P. jirovecii DNA in human bronchoalveolar lavage (BAL) samples. The kit is designed to be used as an aid in the management of cure for the patients with potential P. jirovecii infection in conjunction with all relevant clinical and laboratory ﬁndings.
RTA P. jirovecii Real—Time PCR Kit provides you everything needed for a reliable and efﬁcient detection:
- Very high sensitivity of detection
- Extended linear range
- Complete speciﬁcity for all relevant genotypes and subtypes
- Whole process check by adding the internal control during extraction
- 4 extemal quantitation standards calibrated against the WHO CE marked references
- Compatibility with widely used thermal cyclers
RTA P. jirovecii Real-Time PCR Kit is perfectly designed to amplify and detect a highly conserved region within the P. jirovecii genome by Real Time PCR. It is possible to monitor the nucleic acid amount on-line, during the reaction utilizing hydrolysis probe method in which ﬂuorescence emission increases proportionally to the DNA amount.
Sample Preparation: Nucleic acid extraction is a decisive step prior to qPCR since; providing high yields of nucleic acid and removal of PCR inhibitors in turn improve the ampliﬁcation efﬁciency and critical for avoiding false negative PCR results.
RTA P.jirovecii Real—Time PCR Kit has been validated for use with RTA Genomic DNA Isolation Kit from BAL samples for its best performance.