Detail
RTA Entamoeba histolytica—dispar—moshkovskii Multiplex Real—Time PCR Kit is a multiplex in vitro nucleic acid amplification assay for the detection of Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii bacterial DNA in human gaita samples. The kit is designed to be used as an aid in the management of cure for patients with potential infection in conjunction with all relevant clinical and laboratory findings.
RTA Entamoeba histolytica—dispar—moshkovskii Multiplex Real—Time PCR Kit provides you everything needed for a reliable and efficient detection:
- Very high sensitivity of detection
- Complete specificity for all relevant genotypes and subtypes
- Whole process check by adding the internal control during extraction
- 3 positive controls calibrated against ATCC certified reference materials
- Compatibility with widely used thermal cyclers
RTA E. histolytica 8 E. dispar 8 E. moshkovskii Multiplex Real-Time PCR Kit is perfectlydesigned to amplify and detect highly conserved regions within the E. histolytica / E. dispar / E. moshkovskii genomes by Real Time PCR. It is possible to monitor the nucleic acid amount on-line, during the reaction utilizing hydrolysis probe method in which fluorescence emission increases proportionally to the DNA amount.
Sample Preparation: Nucleic acid extraction is a decisive step prior to qPCR since; providing high yields of nucleic acid and removal of PCR inhibitors in turn improve the amplification efficiency and critical for avoiding false negative PCR results.
RTA E. histolytica 8 E. dispar 8 E. moshkovskii Real-Time PCR Kit has been validated for use with RTA DNA Isolation Kit from Stool for its best performance.
