Detail
RTA E. histolytica Real—Time PCR Kit is an in vitro nucleic acid amplification assay for thequantitation of E. histolytica DNA in human gaita samples. The kit is designed to be used as an aid in the management of cure for the patients with potential E. histolytica infection in conjunction with all relevant clinical and laboratory findings.
RTA E. histolytica Real—Time PCR Kit provides you everything needed for a reliable and efficient detection:
- Very high sensitivity of detection
- Extended linear range
- Complete specificity for all relevant genotypes and subtypes
- Whole process check by adding the internal control during extraction
- 4 external quantitation standards calibrated against the ATCC
- certified reference materials
- Compatibility with widely used thermal cyclers
RTA E. histolytica Real—Time PCR Kit is perfectly designed to amplify and detect a highly conserved region within the E. histolytica genome by Real Time PCR. It is possible to monitor the nucleic acid amount online, during the reaction utilizing hydrolysis probe method in which fluorescence emission increases proportionally to the DNA amount.
Sample Preparation: Nucleic acid extraction is a decisive step prior to qPCR since; providing high yields of nucleic acid and removal of PCR inhibitors in turn improve the amplification efficiency and critical for avoiding false negative PCR results.
RTA E. histolytica Real—Time PCR Kit has been validated for use with RTA DNA Isolation Kit from Stool samples for its best performance.
